Abstract
The cornea is essential for proper ocular function, yet its histological structure varies considerably among animal species. Of particular importance are the palisades of Vogt in the limbal region, as they serve as a niche for limbal epithelial stem cells involved in corneal epithelial regeneration. This study was conducted on 73 eyeballs collected from 18 species of non-human primates originating from the Wrocław Zoological Garden (Poland). Eyeballs were fixed, processed, and embedded in paraffin. Four-micrometer sections were stained with Mayer's H&E and PAS. Microscopically, the cornea showed either a four-layered pattern (anterior corneal epithelium, corneal stroma, Descemet's membrane, posterior corneal epithelium) or a five-layered pattern when Bowman's layer was present. A four-layered cornea occurred in the ring-tailed lemur, gray mouse lemur, Guianan squirrel monkey, Angolan colobus, and L'Hoest's monkey, while the remaining species showed a five-layered structure with Bowman's layer. The anterior corneal epithelium varied between species in thickness and number of cell layers (central region: 2-3 to 10-15 layers; 11.81 ± 0.43 µm to 44.23 ± 0.69 µm; peripheral region: 4-5 to 9-11 layers; 8.63 ± 2.57 µm to 42.45 ± 8.61 µm). Bowman's layer ranged from 1.18 ± 0.01 µm to 3.22 ± 0.05 µm. The corneal stroma thickness differed markedly (237.96 ± 9.64 µm to 1438.29 ± 16.38 µm), as did Descemet's membrane (4.92 ± 0.20 µm to 43.45 ± 0.49 µm), along with PAS reaction intensity. In the limbus, palisades of Vogt ranged from weakly to clearly developed; well-defined crypt-like structures were observed in the red-bellied lemur, red ruffed lemur, black-and-white ruffed lemur, Guianan squirrel monkey, L'Hoest's monkey, Celebes crested macaque, and yellow baboon. The limbal epithelium also varied in thickness (5-6 to 15-17 cell layers). These results confirm distinct species-specific differences in corneal and limbal morphology that may reflect ecological conditions and functional adaptation. The presented data provides a comparative reference for veterinary ophthalmology and for studies on corneal epithelial regeneration involving limbal stem cells.