Abstract
Common peroxidase action and haloperoxidase action are quantifiable as light emission from dioxygenation of luminol (5-amino-2,3-dihydrophthalazine-1,4-dione). The velocity of enzyme action is dependent on the concentration of reactants. Thus, the reaction order of each participant reactant in luminol luminescence was determined. Horseradish peroxidase (HRP)-catalyzed luminol luminescence is first order for hydrogen peroxide (H(2)O(2)), but myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are second order for H(2)O(2). For MPO, reaction is first order for chloride (Cl(-)) or bromide (Br(-)). For EPO, reaction is first order for Br(-). HRP action has no halide requirement. For MPO and EPO, reaction is first order for luminol, but for HRP, reaction is greater than first order for luminol. Haloperoxidase-catalyzed luminol luminescence requires acidity, but HRP action requires alkalinity. Unlike the radical mechanism of common peroxidase, haloperoxidases (XPO) catalyze non-radical oxidation of halide to hypohalite. That reaction is second order for H(2)O(2) is consistent with the non-enzymatic reaction of hypohalite with a second H(2)O(2) to produce singlet molecular oxygen ((1)O(2)*) for luminol dioxygenation. Alternatively, luminol dehydrogenation by hypohalite followed by reaction with H(2)O(2) yields dioxygenation consistent with the same reaction order. Haloperoxidase action, Cl(-), and Br(-) are specifically quantifiable as luminol luminescence in an acidic milieu.