Abstract
Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Although the importance of magnesium (Mg(2+)) ion on SA production has been evident from our previous studies, the role of Mg(2+) ion remains largely unexplored. In this study, we investigated the impact of Mg(2+) ion on SA production and developed a hyper-SA producing strain of M. succiniciproducens by reconstructing the Mg(2+) ion transport system. To achieve this, optimal alkaline neutralizer comprising Mg(2+) ion was developed and the physiological effect of Mg(2+) ion was analyzed. Subsequently, the Mg(2+) ion transport system was reconstructed by introducing an efficient Mg(2+) ion transporter from Salmonella enterica. A high-inoculum fed-batch fermentation of the final engineered strain produced 152.23 ± 0.99 g/L of SA, with a maximum productivity of 39.64 ± 0.69 g/L/h. These findings highlight the importance of Mg(2+) ions and transportation system optimization in succinic acid production by M. succiniciproducens.