The effect of different whitening mouthwashes and simulated tooth brushing on surface roughness and microhardness of nanohybrid resin composite: an in vitro study

不同美白漱口水和模拟刷牙对纳米混合树脂复合材料表面粗糙度和显微硬度的影响:一项体外研究

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Abstract

BACKGROUND: This study investigated the effects of whitening mouthwashes containing active ingredients on a nanohybrid resin composite's surface roughness (Ra) and Vickers microhardness (VHN) under distinct brushing conditions. METHODS: Fifty-four disc-shaped specimens (10 mm diameter × 2 mm thickness) were fabricated and randomly assigned to three experimental conditions (n = 18): distilled water (Control), Patent Blue V-containing mouthwash (Colgate Optic White, COW), and activated charcoal-containing mouthwash (Colgate Plax White + Charcoal, CPWC). Each group was subdivided into brushed and non-brushed subgroups (n = 9). Non-brushed specimens were immersed in 20 mL of the assigned solution at 37 °C for 6 h (T1) and 12 h (T2). Brushed specimens underwent 5 000 and 10 000 brushing cycles before immersion at T1 and T2, respectively. Surface roughness was measured using a contact profilometer, and microhardness was assessed with a Vickers hardness tester. Data were analyzed using three-way mixed ANOVA, one-way ANOVA, repeated measures ANOVA, and Bonferroni correction (α = 0.05). RESULTS: Significant differences were observed in Ra values based on mouthwash type and brushing (p < 0.05), while VHN remained unaffected (p > 0.05). In non-brushed specimens, CPWC exhibited the highest Ra increase (31.2%, + 0.296 μm), significantly higher than Control (1.6%, + 0.015 μm, p = 0.001) and COW (5.9%, + 0.055 μm, p = 0.001). Under brushed conditions, CPWC again showed the most significant increase (33.3%,+ 0.311 μm,p = 0.012). Microhardness showed no statistically significant changes across time points or groups (p > 0.05). However, at T2, CPWC (non-brushed) presented the most significant reduction (- 3.38%, - 1.4 VHN), which was significantly lower than Control (+ 1.98%, + 0.8 VHN) and COW (+ 3.3%, + 1.3 VHN) (p = 0.001). A 5.33% increase (+ 2.1 VHN, p = 0.756) was observed in the brushed CPWC group. CONCLUSIONS: Activated charcoal-containing whitening mouthwashes significantly increased surface roughness, particularly under brushing conditions, indicating a synergistic effect of chemical and mechanical wear. Microhardness values remained stable, suggesting the preservation of the internal structure. These findings highlight the need for cautious recommendation of charcoal-based mouthwashes for patients with resin restorations due to potential surface degradation risks.

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