Abstract
Glycans are modified by acidic molecules, including sialic acid, sulfates, phosphates, and glucuronic acid, forming acidic glycans. Among these, sialylated glycans form major components of deuterostome glycomes, and their structure and function have been widely studied. The other acidic glycans, comprising minor components of the glycome, are often overlooked by glycomics and glycoproteomics methods, although they are implicated in conditions such as inflammatory diseases, neurological diseases, and viral infections. Therefore, minor acidic glycans are high-priority targets for glycomics, and analytical techniques focused on minor acidic glycans are being developed. This review examines the methods of enriching the minor acidic glycans from biological glycomes and examines their structure, providing examples of the application of these techniques in biological and clinical samples.