Abstract
BACKGROUND: Gene-environment interaction is related to the prevalence of hypertension, but the impact of genetic polymorphisms on hypertension may vary due to different geography and population. OBJECTIVE: To explore the impact of the interaction among occupational stress and MTHFR gene and SELE gene polymorphism on the prevalence of hypertension in Xinjiang oil workers. METHODS: A case-control study was conducted on 310 oil workers. In an oilfield base in Karamay City, Xinjiang, 155 hypertensive patients aged 18~60 years old with more than one year of service were selected as the case group, and 155 oil workers without hypertension were selected as the control group according to the 1:1 matching principle (matching conditions: the gender and shift were the same. The age is around 2 years old). The Occupational Stress Scale was used to evaluate the degree of occupational stress, PCR technique was used to detect MTHFR and SELE gene polymorphism, Logistic regression analysis was used to analyze the effects of gene and occupational stress on hypertension, and gene-gene and gene-environment interactions were analyzed by generalized multi-factor dimension reduction method. RESULTS: The G98T polymorphism of SELE gene (χ(2) = 6.776, P = 0.034), the C677T (χ(2) = 7.130, P = 0.028) and A1298C (χ(2) = 12.036, P = 0.002) loci of MTHFR gene and the degree of occupational stress (χ(2) = 11.921, P = 0.003) were significantly different between the case group and the control group. The genotypes GT at the G98T polymorphism of the SELE gene (OR = 2.151, 95% CI [1.227-3.375]), and the dominant model (AC/CC vs AA, OR = 1.925, 95% CI [1.613-3.816]); AC and CC at the A1298C polymorphism of the MTHFR gene (OR (AC) = 1.917, 95% CI [1.064-3.453]; OR (CC) = 2.233, 95% CI [1.082-4.609]), the additive model (CC vs AA, OR = 2.497, 95% CI [1.277-4.883]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200-3.373]); at the C677T polymorphism of the MTHFR gene CT and TT (OR (CT) = 1.913, 95% CI [1.085-3.375]; OR (TT) = 3.117, 95% CI [1.430-6.795]), the additive model (CC vs AA, OR = 1.913, 95% CI [1.085-3.375]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200-3.373]), which could increase hypertension risk (P < 0.05). The gene-gene interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene, and the gene-occupational stress interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene and the occupational stress. CONCLUSION: The interaction of gene mutation and occupational stress in Xinjiang oil workers maybe increase the risk of hypertension.