Abstract
Chemosensory proteins (CSPs) are small soluble proteins found predominantly in insects, with a conserved structure that contains a hydrophobic cavity. While originally associated with chemosensation, they were soon implicated to several other functions related to their ability to bind hydrophobic molecules. Research in the last decade has shown that CSPs may play a role in insecticide resistance. Several CSP genes are upregulated upon induction by sublethal insecticide doses or are highly expressed in resistant populations. RNA interference of CSP genes can restore susceptibility to insecticides. In vitro binding assays and molecular docking simulations suggest that CSPs can strongly bind to insecticides and can accommodate even large molecules in their hydrophobic cavities. Some cases of CSP overexpression in transgenic insects conferring insecticide resistance are reported. Taken together, these results indicate a role for CSPs in insecticide resistance, presumably through a sequestration mechanism, perhaps in combination with other mechanisms like metabolic resistance. This article reviews the evidence for CSP involvement in resistance and discusses ongoing research in the field.