Abstract
Insects sense intraspecific or interspecific information about the chemical substances in the habitat through the sensitive olfactory system to carry out foraging, mating, oviposition, and other activities. The antennae serve as the primary olfactory organs in insects. The olfactory process involves the participation of many proteins, such as odorant-binding proteins (OBPs) and odorant receptors (ORs), but ORs play a central role in olfactory specificity and sensitivity. The beet webworm, Loxostege sticticalis, is an omnivorous agricultural pest that endangers crops and poses a significant risk to the agricultural and animal husbandry production in northern China. In this study, Illumina sequencing was conducted on the antennal transcriptome of male L. sticticalis trapped by three different sex pheromone blends. A total of 10,320 DEGs were identified, from which 46 candidate olfactory genes were selected for further analysis. These candidate olfactory genes comprise 13 odorant receptors, 6 ionotropic receptors (IRs), 3 gustatory receptors (GRs), 12 odorant-binding proteins, and 13 chemosensory proteins (CSPs). In summary, we analyzed the antennal transcriptome of male L. sticticalis trapped by three different sex pheromone blends and identified several candidate olfactory genes. This discovery offers a foundation for further molecular-level investigations into the olfactory system of L. sticticalis.