T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis

T-钙粘蛋白抑制子宫内膜异位症中子宫内膜基质细胞的侵袭和迁移

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作者:Qinsheng Lu, Yanqing Huang, Jiabao Wu, Yutao Guan, Miaomiao Du, Fenghua Wang, Zhihong Liu, Yali Zhu, Guifang Gong, Huomei Hou, Min Zhang, Joy Yue Zhang, Fen Ning, Lixin Chen, Liwei Wang, Gendie E Lash

Methods

Patients were recruited at the Guangzhou Women and Children's Medical Center to study the expression levels of T-cadherin. The expression of T-cadherin was detected by immunohistochemistry staining and western blot. H-score was used to evaluate the staining intensity of T-cadherin. The correlation between T-cadherin expression levels (H-score) and endometriosis patients' age, stage, lesion size and adhesion was analyzed. Endometrial stromal cells from patients with and without endometriosis were isolated, and cell invasion and migration were detected by transwell assays after T-cadherin overexpression. The expression of vimentin in T-cadherin-overexpressed cells was detected by western blot. After T-cadherin overexpression, the phosphorylation profile of signaling pathway proteins was detected with the Proteome Profiler Human Phospho-Kinase Array Kit. Main

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