Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide

A purification procedure for fish myosin heavy chain is described. The protein was injected into rabbits for production of antibodies. The specificity of the antibodies was determined by immunoblotting. The enzyme-linked immunosorbent assay technique was applied to quantify myosin heavy chain bound to isolated polyribosomes of epaxial muscle from fish.