Abstract
Chondrosarcoma is the second most common bone malignancy in adults, and it is often resistant to traditional chemotherapy and radiation therapy. Permanent implantation of iodine-125 ((125)I) seeds has been explored for the treatment of many types of cancer. In this study, the aim was to investigate the proliferative and microRNA (miRNA) effects of (125)I seeds irradiation on human chondrosarcoma SW1353 cells. First, a new in vitro (125)I seed irradiation model was established, and cell viability and miRNA microarray assays were performed before and after exposure to the (125)I seeds. Cell proliferation was inhibited, and miRNA expression was substantially altered by irradiation exposure. The inhibition of cell proliferation was positively correlated with increased radiation doses, with cells showing the highest total radiation dose 7 days after irradiation. A total of 2549 miRNAs were detected in the SW1353 cells after exposure to 6 Gy of radiation, which included 189 differentially expressed miRNAs (98 upregulated and 91 downregulated). Four miRNAs were found to play important roles in the inhibition of cell proliferation after irradiation exposure, including miR-1224-5p, miR-492, miR-135b-5p, and miR-6839-5p. The target genes of the associated miRNAs mentioned were vascular endothelial growth factor A (VEGFA), C-X-C motif chemokine 12 (CXCL12), mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), and apoptosis facilitator Bcl-2-like protein 14 (BCL2L14). Hence, the mitogen-activated protein kinase signaling pathway may be involved in how chondrosarcoma cells respond to (125)I seed irradiation.