Exosomal miR-214 from endometrial stromal cells inhibits endometriosis fibrosis

子宫内膜基质细胞外泌体 miR-214 抑制子宫内膜异位症纤维化

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作者:Di Wu, Pan Lu, Xue Mi, Jinwei Miao

Methods

The primary ectopic ESCs were transfected with miR-214 mimics. The levels of miR-214, CTGF and fibrotic markers were measured by RT-PCR and Immunohistochemistry. A mouse model of endometriosis was established by ip injection of human ectopic endometrial tissues into nude mice. MiR-214-enriched exosomes were injected into the mice and endometriotic lesions were measured on Day 28. Changes in fibrosis of the endometriotic implants were studied by histopathological staining. Main

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