Abstract
Helicoverpa armigera is one of the key pests affecting processing tomatoes and many other crops. A three-year study was conducted to describe the oviposition preferences of this species on determinate tomato plants (mainly the stratum, leaf, leaflet, and leaf side) and the spatial pattern of the eggs in the field, to form a sequential sampling plan. Eggs were found mainly in the exposed canopy, on leaves a (upper stratum) and b (upper-middle stratum) and significantly fewer eggs on leaf c (middle-lower stratum) below flower clusters. This vertical pattern in the plant was found in all phenological growth stages. The spatial pattern was found to be aggregated, with a trend towards a random pattern at lower densities. A sequential sampling plan was developed, based on Iwao's method with the parameters of Taylor's power law, with minimum and maximum sample size of 20 and 80 sample units (plants), respectively (two leaves/plant). For its validation, operating characteristic (OC) and average sample number (ASN) curves were calculated by means of simulation with independent data sets. The β-error was higher than desirable in the vicinity of the economic threshold, but this sampling plan is regarded as an improvement both in effort and precision, compared with the fixed sample plan, and further improvements are discussed.