Conclusions
Our study provides the first evidence of the advantages of cell culture on decellularized tissues for the investigation of oligodendrocyte differentiation and cell/ECM interactions.
Methods
We investigated the differentiation potential of the OLP6 cell line using decellularized brain tissue as the substrate.
Results
We observed that OLP6 differentiated faster on decellularized brain tissues than on conventional 2D-coated surfaces. The relative mRNA expression levels of CNP, PNP, and MBP as well as CSPGs were increased under 3D culture conditions. Conclusions: Our study provides the first evidence of the advantages of cell culture on decellularized tissues for the investigation of oligodendrocyte differentiation and cell/ECM interactions.