Abstract
BACKGROUND AND AIM: Acid-base disturbances, particularly metabolic acidosis, are common in dogs with advanced renal failure and contribute substantially to morbidity and prognosis. Intermittent hemodialysis (IHD) is increasingly used when conventional therapy fails; however, physiological monitoring parameters for incremental intermittent hemodialysis (i-IHD) in dogs remain poorly defined. Venous blood gas (VBG) analysis offers a safer and more practical alternative to arterial sampling, yet its utility during i-IHD has not been systematically evaluated. This study aimed to characterize longitudinal changes in VBG and hemato-biochemical parameters before and after consecutive i-IHD sessions in dogs with renal failure. MATERIALS AND METHODS: In this prospective observational study, 45 client-owned dogs with severe azotemia (serum creatinine >5 mg/dL) due to acute kidney injury (AKI) stage IV-V or chronic kidney disease (CKD) stage IV were enrolled. All dogs underwent three consecutive i-IHD sessions with stepwise increases in treatment intensity. Venous blood samples were collected immediately before and after each session for VBG analysis, including pH, bicarbonate (HCO(3) (-)), total carbon dioxide (TCO(2)), partial pressures of carbon dioxide (pCO(2)) and oxygen (pO(2)), base excess, anion gap, and cerebral oxygen saturation, along with hemato-biochemical profiling. Dialysis adequacy was assessed using Kt/V, urea reduction ratio, and creatinine reduction ratio. Pre- and post-dialysis values were compared using paired statistical analyses. RESULTS: Dogs exhibited mild-to-moderate metabolic acidosis before i-IHD. Across all sessions, i-IHD produced a consistent and significant correction of acid-base imbalance, evidenced by normalization of pH and marked increases in HCO(3) (-) and TCO(2) (p < 0.01). A modest but significant rise in pCO(2) accompanied bicarbonate repletion, while the anion gap remained within the lower borderline range, indicating non-anion gap metabolic acidosis. Significant reductions in blood urea nitrogen and creatinine (approximately 25%-40% per session) confirmed effective solute clearance, with adequacy indices improving progressively across sessions. Electrolyte abnormalities, particularly hyperkalemia, were effectively corrected. CONCLUSION: I-IHD effectively restores acid-base and biochemical homeostasis in dogs with advanced renal failure. Serial VBG monitoring provides clinically meaningful, session-wise information and represents a practical tool for guiding i-IHD without the risks of arterial sampling.