Does BPA alter steroid hormone synthesis in human granulosa cells in vitro?
BPA 是否会改变人类颗粒细胞体外的类固醇激素合成?
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作者:Abdallah Mansur, Michal Adir, Gil Yerushalmi, Ariel Hourvitz, Hila Gitman, Yuval Yung, Raoul Orvieto, Ronit Machtinger
| 期刊: | Human Reproduction | 影响因子: | 6.000 |
| 时间: | 2016 | 起止号: | 2016 Jul;31(7):1562-9. |
| doi: | 10.1093/humrep/dew088 | 种属: | Human |
| 研究方向: | 细胞生物学 | 细胞类型: | 粒细胞 |
Methods
Culture medium and total RNA extracted from the luteinized granulosa cells were examined for estradiol and progesterone levels as well as mRNA and protein expression of steroidogenesis enzymes, using enzyme immunoassays, real-time PCR and western blots. Main
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