Abstract
BACKGROUND: Experimental neuropathic pain (NP) models were developed from peripheral injuries and are widely used for pain research. However, previously published methods and outcomes in the evaluation of sciatic nerve dysfunction vary widely across each report. In this study, we established the electrophysiological analysis of sensory nerve action potentials (SNAPs) and compound motor action potentials (CMAPs) as objective methods for evaluating sciatic nerve dysfunction and revealed the electrophysiological characteristics of NP mice models. METHODS: We used male C57BL/6 J mice, C57BLKS/J lar- + Leprdb/+Leprdb (db/db) mice, and mouse models of paclitaxel-induced NP and spared nerve injury (SNI) surgery (10 mice in each group). For nerve conduction studies, we used evoked potential/electromyography apparatuses and bipolar electrodes as stimulating and recording electrodes for SNAPs and CMAPs. SNAPs were recorded from the sciatic nerve. For the distal motor latency and CMAPs amplitude measurements, we placed the cathode of the recording electrode on the gastrocnemius muscle. RESULTS: In electrophysiological assessments, the SNI model showed the most severe sensory disturbance in SNAP with allodynia in the sciatic nerve. The db/db model showed moderate sensory disturbance with allodynia, whereas the paclitaxel model showed slight sensory disturbance but also with allodynia. Only the SNI model showed significantly lower amplitudes of CMAPs than those of normal mice, whereas the paclitaxel and db/db models showed sensory disturbance but no loss of motor function. CONCLUSIONS: We focused on the detailed methodology of electrophysiological assessment in experimental NP models. Our method of electrophysiological analysis of SNAPs and CMAPs of the sciatic nerve was well reflected in the pain withdrawal tests performed in each NP model and may be useful for future research of NP in experimental NP models.