Preimplantation human blastocysts release factors that differentially alter human endometrial epithelial cell adhesion and gene expression relative to IVF success
植入前人类囊胚释放的因子会差异性地改变人类子宫内膜上皮细胞粘附和基因表达,与 IVF 成功率有关
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作者:C Cuman, E M Menkhorst, L J Rombauts, S Holden, D Webster, M Bilandzic, T Osianlis, E Dimitriadis
| 期刊: | Human Reproduction | 影响因子: | 6.000 |
| 时间: | 2013 | 起止号: | 2013 May;28(5):1161-71. |
| doi: | 10.1093/humrep/det058 | 种属: | Human |
| 研究方向: | 信号转导 | 细胞类型: | 上皮细胞 |
| 信号通路: | Adhesion/ECM | |
Methods
We used real-time RT-PCR arrays containing 84 genes associated with the epithelial to mesenchymal transition. We validated selected genes by real-time RT-PCR (n = 3) and immunohistochemistry in the human endometrium (n = 46). Adhesion assays were performed using HEECs and a trophoblast cell line (n = 3). Main
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