Abstract
Sodium-bicarbonate cotransport in retinal glial cells was studied in the everted eyecup preparation of the rat. Intracellular pH was monitored with the indicator dye BCPCF and fluorescence confocal microscopy. Raising the K+ concentration from 3 to 12 mM in HCO3- -buffered perfusate evoked an intracellular alkalinization in both astrocytes and Müller cells. The alkalinization developed more rapidly and was larger in astrocytes. The K+ -induced alkalinization was HCO3- -dependent; it was reduced by 33% in astrocytes and 71% in Müller cells when HCO3- was removed from the perfusate. The alkalinization was effectively blocked by addition of 0.5 mM 4,4"-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS). Removal of Na+ from the perfusate evoked a rapid acidification in both types of glial cells. The results indicate that astrocytes and Müller cells in situ in the rat retina possess an electrogenic Na+/HCO3- cotransporter.