Abstract
BACKGROUND: The transition from flower to fruit, encompassing flower formation to fruit maturation, has been extensively studied in model plants such as Arabidopsis thaliana. However, the Orchidaceae family, including Vanilla planifolia, exhibits a unique phenomenon known as post-pollination syndrome (PPS), where pollination initiates ovule development but often leads to premature ovary drop. This phenomenon significantly impacts the yield and stability of V. planifolia crops. Understanding the molecular mechanisms underlying PPS is essential for improving crop production. This study explores transcriptomic and histological variations to identify key molecular and phenological changes in the ovary during the flower-to-fruit transition in V. planifolia. RESULTS: The flower-to-fruit transition in Vanilla planifolia involves dynamic changes in gene expression and phenotypic events, which can be categorized into four distinct stages: (1) Pre-pollination: Ovary differentiation is characterized by the enrichment of nitrogen metabolism and photoperiod-responsive pathways. The upregulation of VpVRN5-like and VpNAC14-like suggests their roles in photoperiod-induced flowering and ovarian tissue differentiation in response to nitrate availability. (2) Pollination: Key events include nucellar filament branching and the functional enrichment of pathways associated with growth and responses to light intensity. The upregulation of VpMBS1-like indicates its involvement in regulating and adapting to high light conditions. (3) Post-pollination: This stage is marked by embryo sac formation and pollen tube elongation, with enrichment in auxin response pathways. The upregulation of VpIAA6-like and VpRALF27-like suggests their roles in auxin signaling during ovule development. (4) Fertilization: Seed development is associated with the enrichment of abiotic stress response pathways and carbohydrate transport. The upregulation of VpAAE3-like, VpPR1-like, and VpSWET12-like suggests functions in stress responses and sucrose transport, potentially linked to fungal interactions or symbiosis. CONCLUSIONS: This study characterizes the molecular and phenological changes occurring during the flower-to-fruit transition in V. planifolia by integrating transcriptomic analysis with anatomical data on post-pollination syndrome. Based on functional predictions, this approach provides valuable insights into the mechanisms governing this transition in plants exhibiting PPS and identifies candidate genes for future experimental validation in V. planifolia. CLINICAL TRIAL NUMBER: Not applicable.