Abstract
We present a genome assembly from a specimen of Eunapius fragilis (fragile freshwater sponge; Porifera; Demospongiae; Spongillida; Spongillidae). The genome sequence has a total length of 218.91 megabases. Most of the assembly (99.98%) is scaffolded into 23 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 28.5 kilobases in length. Gene annotation of this assembly on Ensembl identified 26,614 protein-coding genes. Additionally, eight high-quality bacterial metagenomes belonging to the Bacteroidota and Pseudomonadota phyla were assembled.