Abstract
Contamination of soil with lead (Pb) and salinity is a substantial concern, impacting plant health and yield. This research analyzes the synergistic use of Broussonetia papyrifera-derived biochar (BP-BC) and selenium nanoparticles (SeNPs) to alleviate the detrimental impacts of salinity and Pb on barley plants. Field trials in Pb-polluted saline soils showed the use of SeNPs (0, 10 and 20 mg L(-1)) and BP-BC (0, 5 and 10 t ha(-1)) markedly enhanced soil physicochemical attributes, such as soil ESP, pH and EC. The synergistic use of SeNPs and BP-BC substantially alleviated influences of salinity and Pb on barley plant and soil. BP-BC (10t-ha(-1)) diminished pH of soil by 5.98% and exchangeable sodium percentage (ESP) by 29.71% relative to the control group, while enhancing microbial biomass (49.11%). SeNPs (20 mg L(-1)) diminished Pb buildup in barley shoots by 60.39% and in seed by 49.40%, whereas combination of SeNPs and BP-BC resulted in reductions of 73.88% and 59.89%, respectively. These amendments increased activity antioxidant enzyme, with APX by 55.21% and (CAT) rising by 36.30%. The yield of barley grain rose by 39.11% with sole BP-BC application and by 80.20% with combination treatment of SeNPs and BP-BC, indicating their efficacy as environmentally sustainable soil supplements for Pb-polluted and saline soils. These findings highlight efficacy of SeNPs and BP-BC as environmentally benign additives for enhancing barley yield in polluted soils, presenting a viable approach for the sustainable agriculture in saline regions.