Abstract
Topological defects are singularities within a field that cannot be removed by continuous transformations. The definition of these irregularities requires an ordered reference configuration, calling into question whether they exist in disordered materials, such as glasses. However, recent work suggests that well-defined topological defects emerge in the dynamics of glasses, even if they are not evident in the static configuration. In this study, we reveal the presence of topological defects in the vibrational eigenspace of a two-dimensional experimental colloidal glass. These defects strongly correlate with the vibrational features and spatially correlate with each other and structural "soft spots", more prone to plastic flow. This work experimentally confirms the existence of topological defects in disordered systems revealing the complex interplay between topology, disorder, and dynamics.