Adoptive Transfer of Basophils Enriched from Mouse Spleen

小鼠脾脏富集嗜碱性粒细胞的过继转移

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作者:Adrian M Piliponsky ,Stephen J Galli

Abstract

CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4+ T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b+ cells obtained from mouse spleens by magnetic enrichment. This protocol can be used to assess the contribution of basophils or basophil-derived mediators to a certain immune response.

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