Abstract
Quantitative image analysis turns microscopic data that might otherwise be merely descriptive into reliable mechanistic information. This unit provides an overview of the types of analysis that have been useful in the past, as well as descriptions of emerging applications. In addition, two protocols are included for importing data and for the first steps of data manipulation in the more common analytical applications of the freeware, NIH Image and ImageJ, as well as commercially available imaging software, Adobe Photoshop.