Abstract
This unit describes the infection of mice and guinea pigs with mycobacteria via the aerogenic route, or of mice via the intravenous route, as well as necropsy methods for the determination of mycobacterial loads within target organs. Additionally, methods for cultivating mycobacteria and preparing stocks are described. The protocols outlined are primarily used for M. tuberculosis, but can also be used for the study of other opportunistic Mycobacterial species.