The study of progesterone action in human myometrial explants

孕酮在人子宫肌层外植体中的作用研究

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作者:E X Georgiou, K Lei, P F Lai, A Yulia, B R Herbert, M Castellanos, S T May, S R Sooranna, M R Johnson

Methods

Myometrial biopsies obtained at Caesarean section were dissected into explants after a portion was immediately snap frozen (t = 0). Microarray analysis was used to compare gene expression of t = 0 with paired (i) explants, (ii) passage 4 myometrial cell cultures or (iii) the hTERT myometrial cell line. Western blotting and chemokine/cytokine assays were used to study P4 signalling in myometrial explants. Main

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