A pH tuning single fluorescent probe based on naphthalene for dual-analytes (Mg(2+) and Al(3+)) and its application in cell imaging

基于萘的pH调节单荧光探针用于双分析物(Mg(2+)和Al(3+))检测及其在细胞成像中的应用

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Abstract

In this study, a naphthalene Schiff-base P which serves as a dual-analyte probe for the quantitative detection of Al(3+) and Mg(2+) has been designed. The proposed probe showed an ''off-on'' fluorescent response toward Al(3+) in ethanol-water solution (1 : 9, v/v, pH 6.3, 20 mM HEPES) over other metal ions and anions, while the detection by the probe could be switched to Mg(2+) by regulating the pH from 6.3 to 9.4. The sensing mechanisms of P to Al(3+)/Mg(2+) are attributed to inhibition of the photo-induced electron transfer (PET) process by the formation of 1 : 1 ligand-metal complexes. More importantly, the probe was applied successfully in living cells for the fluorescent cell-imaging of Al(3+) and Mg(2+).

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