Abstract
Cytoplasmic vacuolization is a cellular morphological alteration characterized by the presence of substantial vacuole-like structures originating from various cellular organelles. This phenomenon is often observed in various anticancer treatments, including chemotherapeutic drugs, and photodynamic therapy (PDT), and is frequently linked with cell death. Nevertheless, the precise mechanisms underlying cytoplasmic vacuolization and ensuing cell death remain ambiguous. Cytoplasmic vacuolization associated cell death (CVACD) is a complex process characterized by cellular stress, encompassing ER stress, heightened membrane permeability, ion imbalance, and mitochondrial dysfunction. The MAPK signaling pathway is closely associated with the activation of CVACD. This review provides a thorough examination of contemporary studies on cytoplasmic vacuolization in mammalian cells, elucidating its etiology, origins, and molecular pathways. Additionally, it highlights the potential of CVACD as an innovative therapeutic strategy for cancer.