Molecular identification of resistance to organophosphates and carbamates in Aedes aegypti of different physiological ages in a cemetery in Peru

在秘鲁一处墓地,对不同生理年龄的埃及伊蚊进行有机磷酸酯和氨基甲酸酯类杀虫剂抗性的分子鉴定

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Abstract

In 2024, a cross-sectional, quantitative, and descriptive study was conducted to molecularly identify resistance to organophosphates and carbamates in Aedes aegypti of different physiological ages in a cemetery in northern Peru. Female specimens were captured, taxonomically identified, their ovaries dissected, and the parity percentage calculated. Likewise, the legs of nulliparous, parous, and gravid females were dissected, DNA was extracted, and PCR was performed to detect the mutant ACEr gene associated with resistance to organophosphates and carbamates. A total of 218 (100%) females were captured, with a nulliparity index of 37,61% and a parity index of 62,39%, of which 13,23% of parous females were gravid at the time of capture. The ACEr gene was detected in all three physiological age groups. It is inferred that in the analyzed cemetery, there are Aedes aegypti populations carrying the genetic mutation conferring resistance to organophosphate and carbamate insecticides.

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