Mimicry in the Bite: Shared Sequences Between Aedes aegypti Salivary Proteins and Human Proteins

叮咬中的拟态:埃及伊蚊唾液蛋白与人类蛋白的共享序列

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Abstract

BACKGROUND: Molecular mimicry contributes to the development of unwanted responses to self-antigens. Autoimmune phenomena have been observed in diseases caused by Aedes aegypti-transmitted arboviruses, but the occurrence of mimicry between salivary and human proteins has been unexplored. METHODS: We used bioinformatic tools to determine if peptides from Aedes aegypti salivary proteins were present in the human proteome. We further characterized the potential of shared sequences to induce immunity by analyzing their predicted binding to MHC molecules and their occurrence in peptides from the Immune Epitope Database (IEDB). RESULTS: We analyzed 9513 octapeptides from 29 Aedes aegypti salivary proteins against the human proteome and found 47 peptides identical to sequences from 52 human proteins, ranging in length from 8 to 18 amino acids. We found 302 matches of peptides predicted to bind with high affinity to MHC-I and MHC-II alleles associated with autoimmune diseases, and 14 human peptides containing shared sequences with Aedes aegypti salivary proteins validated as immunogenic in the IEDB. CONCLUSIONS: These results support the existence of molecular mimicry between Aedes aegypti salivary proteins and human antigens and provide a framework for studies to determine its contribution to responses directed to self-antigens in the context of arboviral infections.

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