Abstract
Anaerobic ammonium oxidation (anammox) is an energy-efficient technology for wastewater nitrogen removal. However, the byproduct nitrate has hindered development and application of anammox process. Meanwhile, the knowledge of nitrate formation during anammox process is insufficient, which prohibits high nitrogen removal. This review firstly summaries and discusses valuable findings on nitrate formation, including molecular mechanism of nitrate production, microbial pathway of nitrate reduction and its net formation. Specially, influences of operating conditions on mechanisms and patterns of nitrate formation are analyzed. Then, based on nitrate formation mechanism, current strategies of nitrate removal from anammox process are reevaluated. Finally, the key knowledge gaps and further process development are presented. Overall, this review sheds light on the understanding of nitrate formation of anammox process, which would further facilitate and optimize the process design and operation for high performance nitrogen removal.