Naming internal insertion alleles created using CRISPR in Saccharomyces cerevisiae

利用 CRISPR 技术在酿酒酵母中创建的内部插入等位基因的命名

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Abstract

The budding yeast Saccharomyces cerevisiae is a powerful model organism, partly because of the ease of genome alterations due to the combination of a fast generation time and many molecular genetic tools. Recent advances in CRISPR-based systems allow for the easier creation of alleles with internally inserted sequences within the coding regions of genes, such as the internal insertion of sequences that code for epitopes or fluorescent proteins. Here we briefly summarize some exisiting nomenclature standards and suggest nomenclature guidelines for internal insertion alleles which are informative, consistent, and computable.

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