Abstract
Visualization is widely used to help explore and interpret high dimensional single cell (sc) omics data, such as scRNA-seq expression data. In particular, uniform manifold approximation and projection (UMAP) has become nearly ubiquitous in scientific publications that apply single cell omics technologies. Some experts have expressed concerns that the global cell-cell relationship, especially the spatial distances among cell clusters in a dataset, may not be faithfully depicted in a 2-dimensional (2D) UMAP. To help users to better appreciate this issue with their own data, we created an online server for the community to upload their single cell data and interactively make UMAPs with different hyper-parameters to witness how the distribution of cell clusters changes. The server thus can help promote proper usages of UMAP, especially to avoid the common pitfalls in misinterpretation of inter-cluster relationships in single cell studies.