Abstract
Dean's flow and Dean's instability have always been important concepts in the inertial microfluidics. Curved channels are widely used for applications like mixing and sorting but are limited to Dean's flow only. This work first reports the Dean's instability flow in high aspect ratio channels on the deka-microns level for [Formula: see text]. A new channel geometry (the tortuous channel), which creates a rolled-up velocity profile, is presented and studied experimentally and numerically along with other three typical channel geometries at Dean's flow condition and Dean's instability condition. The tortuous channel generates a higher De environment at the same Re compared to the other channels and allows easier Dean's instability creation. We further demonstrate the use of multiple vortexes for mixing. The mixing efficiency is considered among different channel patterns and the tortuous channel outperforms the others. This work offers more understanding of the creation of Dean's instability at high aspect ratio channels and the effect of channel geometry on it. Ultimately, it demonstrates the potential for applications like mixing and cell sorting.