Abstract
Acute pancreatitis (AP), a common disease, causes significant morbidity and mortality in clinical practice. Our objective of this study was to establish an experimental mouse AP model with cerulein treatment and to explore the susceptibility of mouse strains on the severity of pancreatic injury and the subsequent repair and regeneration. C57BL/6 and FVB/N mouse strains were used in this study. AP model was induced by six hourly intraperitoneal (i.p.) injections of cerulein dissolved in saline (100 μg/kg) administered on four consecutive days. Animals were sacrificed on 1, 3 and 7 days after last cerulein treatment, and then pancreas tissues were harvested and subjected to various histological, cellular and molecular analysis. Analyses of pancreatic injury and pancreatic amylase expression indicated that this cerulein-induced AP model was established successfully and that FVB/N mice showed more severe pancreatic injury and poor recovery compared to C57BL/6 strain. Analyses of myeloperoxidase (MPO), IL-1β and NF-κB showed that FVB/N strain exhibited more severe inflammation in the pancreas compared to C57BL/6 mice. Immunofluorescence analysis of activated caspase-3 and TUNEL assay indicated that the pancreas of FVB/N strain had more apoptosis compared to C57BL/6 mice. Analysis of Ki67 indicated FVB/N mice experienced more active proliferation compared to C57BL/6 strain. Collectively, these results demonstrated that there exists differential susceptibility on pancreatic injury and regeneration between FVB/N and C57BL/6 mice in the cerulein-induced AP.