Abstract
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. K(m) values were: adenosine triphosphate (ATP), 6.25 x 10(-4)m; formate, 1.67 x 10(-3)m; and tetrahydrofolate, 1.00 x 10(-3)m; at pH 8.0. There was a requirement for divalent metals and specifically for ATP.