Abstract
BACKGROUND: Bacteremia due to viridans group streptococci (VGS), particularly Streptococcus mitis-oralis group (SMOG), is common in neutropenic patients after hematopoietic cell transplantation (HCT) despite use of fluoroquinolone (FQ) prophylaxis. VGS bloodstream isolates in patients receiving FQ prophylaxis are typically FQ-resistant (FQ-R). This study assessed the prevalence of colonization with FQ-R SMOG prior to HCT and the risk of VGS bacteremia during neutropenia. [Figure: see text] [Figure: see text] METHODS: This is an interim report of a prospective observational study enrolling patients undergoing HCT at a New York City hospital from June 2024 to February 2025. We collected buccal, oropharyngeal, and perianal swabs (ESwabs) during the week prior to HCT. Swabs underwent selective culture by direct plating on Columbia nalidixic acid agar with levofloxacin (LVX) disks and by broth enrichment (tryptic soy broth with 2 μg/mL of LVX and 4 μg/mL of colistin) before plating. LVX resistance was confirmed by disk diffusion and species identification was determined by matrix-assisted laser desorption ionization–time of flight mass spectrometry. Patients received LVX prophylaxis during neutropenia and were followed until neutrophil recovery or up to 30 days. RESULTS: Among the first 38 patients enrolled, 35 (92%) underwent allogeneic HCT and 23 (61%) had received FQs within 3 months of enrollment (Table 1). Prior to HCT, 27 (71%) were colonized with FQ-R SMOG, and an additional 7 (18%) of remaining patients were colonized with FQ-R VGS species other than SMOG. Four (15%) patients with FQ-R SMOG colonization developed VGS bacteremia (all due to SMOG) compared to none without FQ-R SMOG colonization (Figure 1; p=0.3). Median time from HCT to VGS bacteremia was 6 days (interquartile range [IQR] 6-9). Compared to FQ-R SMOG-colonized patients without VGS bacteremia, those with VGS bacteremia were more likely to have previously lost ≥5 teeth (p=0.013) and had a longer median duration of neutropenia (21 vs 14 days; p=0.033). CONCLUSION: Colonization with FQ-R SMOG prior to HCT is common. In the setting of FQ prophylaxis, VGS bacteremia occurs primarily in colonized patients and is likely caused by their colonizing FQ-R strain. Screening for FQ-R SMOG prior to HCT may identify patients at risk of VGS bacteremia despite FQ prophylaxis. DISCLOSURES: Markus Plate, MD, F2G: Grant/Research Support|Gilead: Grant/Research Support|Merck: Grant/Research Support John Lee, MD, Astellas: Honoraria|BioFire Diagnostics, LLC: Grant/Research Support|Calliditas: Travel support|Eurofins Viracor: Patent US-2020-0048713-A1 licensed to Eurofins Viracor Lars Westblade, PhD, Elements Materials Technology: Grant/Research Support|Hardy Diagnostics: Grant/Research Support|Melinta Therapeutics: Grant/Research Support|Selux Diagnostics: Grant/Research Support|Shionogi: Advisor/Consultant|SNIPRBIOME: Grant/Research Support Michael J. Satlin, MD, MS, AbbVie: DSMB participant|bioMerieux: Grant/Research Support|Merck: Grant/Research Support|SNIPRBiome: Grant/Research Support