Abstract
Colorectal cancer (CRC) is a common malignant tumor worldwide, and its tumor microenvironment (TME) plays a crucial role in tumor progression. Neutrophil extracellular traps (NETs), as an important component of the TME, have received widespread attention in recent years. This article explores the biological functions and molecular mechanisms of NETs in CRC and their impact on disease progression, while analyzing the application of single-cell sequencing technology (SCS) in this field. The development of SCS provides a new perspective for understanding the role of NETs in CRC. By combining SCS technology, targeting key regulatory nodes of NETs is expected to reverse the immunosuppressive microenvironment and provide a theoretical basis for developing novel diagnostic biomarkers and targeted therapeutic strategies, thereby promoting the development of precision medicine in CRC and helping enhance patient prognosis. Future research should further explore the integration of SCS technology with complementary methodologies to investigate NETs and develop specific detection methods and therapeutic strategies targeting NETs to enhance early diagnosis and treatment efficacy of tumors.