Abstract
Spatial context is becoming increasingly important in the omics disciplines. Spatial proteomics is a diverse field encompassing numerous techniques that provide both the location and identity of proteins in biological samples. Improving upon bulk analyses, spatial proteomics can map peptides and intact proteins within tissue. This review focuses on the application of matrix-assisted laser desorption/ionization (MALDI) in spatial proteomics. Approaches are grouped into two general categories and discussed: protein MALDI MSI and MSI-guided spatial proteomics. A discussion of the workflow for each method is presented, and challenges to each approach are discussed. Recent and technically interesting cases in the literature are presented for each category. This review aims to guide researchers interested in MALDI protein imaging through the strengths, weaknesses, and technical considerations of the many workflows available to them.