Abstract
CF-19 Proteomics and mass spectrometry have provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics and modifications affect cellular processes and how cellular processes and the environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry to UF scientists and students. Here we present our capabilities in proteomics and other analytical services. The tools include a gel-based 2D-DIGE (Two Dimentional Difference Gel Electrophoresis) and gel-free iTRAQ (Isobaric Tags for Relative and Absolute Quantitation). Along with our capacity of separating thousands of proteins and characterizing differential protein expression, we have a suite of state-of-the-art mass spectrometers available for biomedical sciences and advanced technology research, including a tandem time-of-flight (4700 Proteomics Analyzer, AB), quadrupole/time-of-flight (QSTAR XL, AB), and hybrid quadrupole-linear ion-trap (4000 QTRAP, AB). These instruments are mainly used for protein identification, posttranslational modification characterization and protein expression analysis (e.g., Mass Western). Our facility is also set up to provide Edman de novo N-terminal protein sequence analysis and Biacore biomolecule interaction analysis. We are fully set up to synthesize and purify peptides and have a good track record with this service as well. Proteomics and mass spectrometry are useful in large-scale suvey of proteome for hypothesis generation as well as in detailed analysis of target proteins for hypothesis testing. Our services also include accurate molecular weight analysis, MRM-based protein screening and targeted metabolite profiling. To ensure success and maximize productivity, the facility offers education, consultation, data processing and reporting, and support of grant application.