Abstract
BACKGROUND: Single-domain antibody fragments (aka V(H)H, ~ 13 kDa) are promising delivery systems for brain tumor theranostics; however, achieving efficient delivery of V(H)H to intracranial lesions remains challenging due to the tumor-brain barrier. Here, we evaluate low-dose whole-brain irradiation as a strategy to increase the delivery of an anti- human epidermal growth factor receptor type 2 (HER2) V(H)H to breast cancer-derived intracranial tumors in mice. METHODS: Mice with intracranial HER2-positive BT474BrM3 tumors received 10-Gy fractionated cranial irradiation and were evaluated by noninvasive imaging. Anti-HER2 V(H)H 5F7 was labeled with (18)F, administered intravenously to irradiated mice and controls, and PET/CT imaging was conducted periodically after irradiation. Tumor uptake of (18)F-labeled 5F7 in irradiated and control mice was compared by PET/CT image analysis and correlated with tumor volumes. In addition, longitudinal dynamic contrast-enhanced MRI (DCE-MRI) was conducted to visualize and quantify the potential effects of radiation on tumor perfusion and permeability. RESULTS: Increased (18)F-labeled 5F7 intracranial tumor uptake was observed with PET in mice receiving cranial irradiation, with maximum tumor accumulation seen approximately 12 days post initial radiation treatment. No radiation-induced changes in HER2 expression were detected by Western blot, flow cytometry, or on tissue sections. DCE-MRI imaging demonstrated transiently increased tumor perfusion and permeability after irradiation, consistent with the higher tumor uptake of (18)F-labeled anti-HER2 5F7 in irradiated mice. CONCLUSION: Low-level brain irradiation induces dynamic changes in tumor vasculature that increase the intracranial tumor delivery of an anti-HER2 V(H)H, which could facilitate the use of radiolabeled V(H)H to detect, monitor, and treat HER2-expressing brain metastases.