Abstract
PURPOSE: The present retrospective observational study was designed to explore the expression deregulation of exosomal microRNAs, hsa-miR-144-3p, hsa-miR-4262, and related genes in brain tumor patients. METHODS: Exosomes were extracted from 400 brain tumor patients and 400 controls and characterized using DLS, TEM, and ELISA. Quantitative PCR was used to check the expression level of hsa-miR-144-3p, hsa-miR-4262, phosphatidylinositol-3-kinase (PI3K) and AKT kinase (AKT) in study cohort. CMV level in brain tumor patients was checked by quantifying UL83. RESULTS: Data analysis showed significant deregulation of hsa-miR-144-3p (p < 0.0001), hsa-miR-4262 (p < 0.0001), PI3K (p < 0.0001), and AKT (p < 0.0001) in brain tumor patients. Conclusions: ROC curve analysis showed that deregulation of hsa-miR-144-3p, hsa-miR-4262, PI3K, and AKT may act as good diagnostic markers for brain tumor patients.