Metabolic regulation and quality enha2ancement mechanisms in dendrobium officinale flower tea aroma-crafting processes revealed by fuzzy mathematics and metabolomics

模糊数学和代谢组学揭示石斛花茶香气塑造过程中的代谢调控和品质提升机制

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Abstract

In this study, the effects of aroma-crafting processes on the quality of Dendrobium officinale flower tea were evaluated using fuzzy mathematics and metabolomics. Among the tested ratios, the 7:3 flower-to-tea ratio achieved the highest heap temperature (45.2°C) and demonstrated superior aroma adsorption and release. Sensory analysis revealed a significantly higher fuzzy membership score for the 7:3 ratio (peak value 0.37), indicating the best overall sensory quality. After processing, amino acid content increased from 2.21% to 2.51%, while tea polyphenols decreased from 1.91% to 1.00%, improving the tea's taste. GC-MS identified 65 volatile compounds, with phenylethanol (27.88%), 2-methoxy-4-vinylphenol (20.97%), and phenylacetaldehyde (3.65%) being most prominent during the floral enhancement stage. Metabolomics revealed 235 significantly differential non-volatile metabolites and enrichment of flavonoid and unsaturated fatty acid biosynthesis pathways. These results provide quantitative insights into optimizing aroma-crafting to enhance tea quality.

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