Abstract
This study investigates the metabolic changes induced by endurance exercise, specifically race walking, in a cohort of 19 athletes. Blood samples were collected at four time points: pre-exercise (REST), immediately post-exercise (STAT), 3 hours into recovery (REC3), and 22 hours post-exercise (REC22). A total of 859 metabolites were identified through the untargeted method, and 465 metabolites and 411 lipids were identified through the targeted methods. Rigorous quality control measures were implemented throughout the study to ensure data reliability. The comprehensive dataset, which is publicly available on the Metabolomics Workbench website, offers valuable insights into the systemic metabolic shifts triggered by endurance exercise. This resource may prove instrumental in uncovering biomarkers associated with athletic performance, providing a foundation for future research in exercise physiology and metabolic health.