T-RHEX-RNAseq - a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells

T-RHEX-RNAseq - 一种基于标记、rRNA 阻断、随机六聚体引发的 RNAseq 方法,可直接从极少量的裂解细胞中生成链状 RNAseq 文库

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作者:Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert Månsson

Background

RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming - to allow analysis of a broader range of transcripts - remains challenging.

Conclusion

T-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.

Results

We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps.

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