T-RHEX-RNAseq - a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells
T-RHEX-RNAseq - 一种基于标记、rRNA 阻断、随机六聚体引发的 RNAseq 方法,可直接从极少量的裂解细胞中生成链状 RNAseq 文库
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作者:Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert Månsson
| 期刊: | BMC Genomics | 影响因子: | 3.500 |
| 时间: | 2023 | 起止号: | 2023 Apr 17;24(1):205. |
| doi: | 10.1186/s12864-023-09279-4 | 研究方向: | 细胞生物学 |
| 细胞类型: | 其它细胞 | |
Background
RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming - to allow analysis of a broader range of transcripts - remains challenging.
Conclusion
T-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.
Results
We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps.
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