Abstract
β-D-xylosidases (BXLs) are pivotal enzymes in xylan degradation, playing essential roles in plant development and stress responses. In this study, we identified 29 GmBXL genes in soybean through homolog alignment. Phylogenetic analysis classified these genes into three groups, with Group III being legume-specific. The GmBXLs are unevenly distributed across 15 chromosomes, with their expansion driven by both tandem and segmental duplications. Conserved motif and domain analyses revealed functional conservation, particularly in family 3 of glycoside hydrolase domains. Promoter regions of GmBXLs are enriched with hormone-responsive and stress-related cis-elements, indicating their involvement in diverse biological processes. Tissue-specific expression analysis revealed differential GmBXLs expression across leaves, roots, flowers, and seeds, with GmBXL13 and GmBXL26 exhibiting notably high transcript levels in pods and seeds. Under salt stress, 26 GmBXLs exhibited significant expression changes, with 20 genes up-regulated in both leaves and roots, highlighting their roles in salt tolerance. These findings enhance our understanding of the evolutionary and functional characteristics of GmBXLs, providing valuable insights for molecular breeding of salt-tolerant soybean varieties.