Abstract
Vitamin E, mainly encompassing tocopherols and tocotrienols, is an essential antioxidant synthesized in the photosynthetic tissues of plants and photosynthetic bacteria, as well as in certain algae, yet dietary intake often falls short of recommended levels. Although synthetic supplements are available, natural vitamin E demonstrates higher bioavailability, creating a need for biofortification strategies to enrich crops with this nutrient. Recent advances in molecular genetics have elucidated key components of the vitamin E biosynthesis pathway, uncovering complex regulatory mechanisms and expanding opportunities for genetic enhancement. This review integrates current advances in vitamin E biosynthesis, novel gene discovery, diverse biofortification strategies, and insights into transporter-mediated regulation to enhance tocopherol and tocotrienol levels in staple crops. By aligning these advances, this review provides a framework to drive innovative biofortification efforts, positioning vitamin E enrichment as a sustainable solution for improved human and animal health.