Abstract
Dirigent (DIR) proteins are key regulators of lignin and lignan biosynthesis and play critical roles in plant hormone responses, abiotic stress tolerance, and growth and development. This study identified and characterized 47 PeDIR genes in Moso bamboo, classifying them into three groups. Phylogenetic and comparative analyses revealed strong evolutionary conservation, with the Moso bamboo PeDIR genes being most closely related to those in rice and maize. DIR proteins within each subfamily exhibited high conservation in motif composition, domain structure, and 3D configuration. Subcellular localization and protein interaction studies further elucidated PeDIR gene functions. Specifically, PeDIR02 primarily localized to the cell membrane and was shown to be unable to form homodimers in yeast two-hybrid (Y2H) assays. Transcriptome and expression analyses revealed the involvement of PeDIR genes in rapid shoot growth, indicating roles in lignin biosynthesis and cell wall modification. Transcriptome and qRT-PCR data also demonstrated the responsiveness of these genes to hormones and abiotic stresses, such as drought and salinity. This study constructed the first comprehensive regulatory network between transcription factors (TFs) and PeDIR genes, identifying ERF, DOF, and MYB TFs as key synergistic regulators of PeDIR gene expression.