Abstract
Pear is an important originate fruiter in China, ranking first in the world in terms of cultivation area and yield. However, it is susceptible to infection by Alternaria alternata (A. alternata), resulting in a reduction of approximately 30% in yield. While both lignin and salicylic acid (SA) are recognized as key components of plant immune responses, the molecular mechanisms connecting these pathways remain poorly understood. Here, we have discovered a nuclear localization transcription activator PbrMYB14 in pears, whose expression can be induced by exogenous SA and A. alternata. Overexpression of PbrMYB14 significantly increased lignin and SA content in pears, making them more resistant to A. alternata, and the relative lesion area decreased by 68.95% compared with WT plants. By analyzing the transcriptome of PbrMYB14-overexpressing plants, the lignin synthesis gene Pbr4CL1 and SA synthesis gene PbrPAL1 regulated by PbrMYB14 were screened and identified. Through yeast one-hybrid (Y1H) and a Dual-Luciferase assay (LUC), it was confirmed that PbrMYB14 positively regulates the expression of Pbr4CL1 and PbrPAL1 genes. Our results suggest that PbrMYB14 links lignin resistance and SA resistance in pears, providing valuable information for future genetic breeding research on pear disease resistance.